Collect. Czech. Chem. Commun.
1979, 44, 145-147
https://doi.org/10.1135/cccc19790145
Hog pancreatic α-amylase; N-terminal sequence analysis
Ivan Kluh
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
Abstract
Both isozymes of hog pancreatic amylase were digested with chymotrypsin, subtilisin and pronase. These digests were passed over a column of sulfoethyl-Sephadex C-25 and peptides not containing free NH2-groups thus isolated. It was shown that both isozymes have the same N-terminal amino acid sequence, pyrrolidonecarboxylic acid - tyrosine. No peptides with acetylated amino groups were found.
